A method for biotransformation of eugenol to vanillin was developed by Rabenhorst and Hopp (1991, patent application EP0405197), based on a new Pseudomonas sp. We do not retain these email addresses.ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.Institut für Molekulare Mikrobiologie und Biotechnologie der Westfälischen Wilhelms-Universität Münster, D-48149 Münster, GermanyInstitut für Molekulare Mikrobiologie und Biotechnologie der Westfälischen Wilhelms-Universität Münster, D-48149 Münster, GermanyInstitut für Molekulare Mikrobiologie und Biotechnologie der Westfälischen Wilhelms-Universität Münster, D-48149 Münster, GermanyHighly Efficient Biotransformation of Eugenol to Ferulic Acid and Further Conversion to Vanillin in Recombinant Strains of Enter multiple addresses on separate lines or separate them with commas.This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.Highly Efficient Biotransformation of Eugenol to Ferulic Acid and Further Conversion to Vanillin in Recombinant Strains of Isolation, manipulation, analysis, and transfer of DNA.Isolation of total RNA and cloning of vanillyl alcohol oxidase gene PCR-directed mutagenesis for elimination of interfering restriction sites.Cloning and functional expression of the vanillyl alcohol oxidase gene (Biotransformation of eugenol to ferulic acid in 50-ml cultures.Technical-scale production of ferulic acid from eugenol.Two-step biotransformation of eugenol to vanillin with recombinant strains of Molecular Weight 180.20 . acting on paired donors with incorporation of molecular oxygen (1.14)BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERINGMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIAEnzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymesBIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERINGMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIAEnzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymesHydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERINGFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTUREPreparation of oxygen-containing organic compounds containing a hydroxy groupPreparation of oxygen-containing organic compounds containing a hydroxy group aromaticEnzymes for the synthesis of coniferyl alcohol, coniferyl aldehyde, ferulic acid, vanillin, vanillic acid and their applications
pH 6.0: 50% loss of activity within 4 h).In addition to coniferylaldehyde (100%), the enzyme also uses trans-cinnamaldehyde (96.7%), sinapylaldehyde (76.7%), p-anisaldehyde (23.1%), benzaldehyde (17.8) and 3,5-dimethoxy -Benzaldehyde (7.6%) and 3-hydroxybenzaldehyde (1.7%). of synthetic oligonucleotides was developed according to the "primer hopping strategy" 47, 2639-2640) published another article on the first steps in eugenol degradation; this time with a soil isolate known as Also in 1983, Sutherland et al. Based on graphite furnace atomic absorption spectroscopic analysis, it is thought to contain one copper atom per protein molecule.